TABLE 3.

Steady-state kinetic parameters for one-base incorporation opposite guanine O6 adducts by human REV1

Template	dNTP	Km	kcat	kcat/Km	f(misinsertion ratio)
		μm	s-1	mm-1 s-1
G	C	13 ± 2	0.012 ± 0.001	0.92	1
	G	250 ± 50	0.0014 ± 0.0001	0.0056	0.0061
	T	350 ± 60	0.0021 ± 0.0001	0.0060	0.0065
O6-MeG	C	60 ± 10	0.0034 ± 0.0001	0.057	1
	G	190 ± 20	0.000071 ± 0.000001	0.00037	0.0065
	T	360 ± 40	0.00043 ± 0.00002	0.0012	0.021
O6-BzG	C	40 ± 10	0.0083 ± 0.0002	0.21	1
	G	250 ± 60	0.0030 ± 0.0002	0.012	0.057
	T	330 ± 50	0.0012 ± 0.0001	0.0036	0.017
O6-PobG	C	120 ± 30	0.000018 ± 0.000001	0.00015	1