TABLE 4.

Comparison of steady-state kinetic parameters for one-base incorporation opposite N2-EtG and N2,N2-diMeG by human REV1

Template: dNTP	Km	kcat	kcat/Km	-Fold difference of kcat/Km compared with N2-EtG	f (misinsertion ratio)
	μm	s-1	mm-1 s-1	-fold
N2-EtG: dCTP	9.4 ± 1.8	0.0046 ± 0.0002	0.49		1
N2,N2-diMeG: dCTP	10 ± 2	0.0080 ± 0.0003	0.80	1.6-fold higher	1
N2-EtG: dGTP	290 ± 90	0.0014 ± 0.0001	0.0048		0.010
N2,N2-diMeG: dGTP	190 ± 50	0.0030 ± 0.0002	0.016	3-fold higher	0.046
N2-EtG: dTTP	400 ± 60	0.0019 ± 0.0001	0.0048		0.010
N2,N2-diMeG: dTTP	140 ± 40	0.0039 ± 0.0003	0.028	6-fold higher	0.080