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Homobifunctional cross-linker and dimeric ActA. A, structural formula for N,N′-bis(iodoacetyl)-bis[2-(3-aminopropoxy)ethyl] ether, a flexible hydrophilic cysteine-reactive cross-linker. B, purified soluble ActA migrates with an apparent mobility of 97 kDa, whereas synthetic ActA dimer migrates with an apparent mobility of 185 kDa by SDS-PAGE. Molecular weight marker positions are indicated to the left of the Coomassie-stained gel.
