FIGURE 1.

H. pylori induces the transcriptional up-regulation of DAF in gastric epithelial cells. MKN28 gastric epithelial cells were pretreated with either actinomycin D (ActD) or cycloheximide (CHX) at the indicated concentrations and then co-cultured with the H. pylori cag⁺ strain J166 for 24 h at an m.o.i. = 100. A, Western blot analysis was performed using an anti-DAF antibody as described under “Experimental Procedures.” -, cells incubated with medium alone. A representative blot is shown. Anti-GAPDH blots served as normalization controls. B, densitometry represents data from three independent experiments. Error bars, S.E. ^*, p < 0.001 J166 versus control cells or ActD-treated cells. §, p < 0.01 J166 versus cycloheximide-treated cells.