FIGURE 2.

The rate of ChREBP nuclear entry is increased in high glucose conditions. a, 832/13 cells were co-transfected with FLAG-tagged ChREBP and Mlx overnight in RPMI medium containing 11 mm glucose and were then incubated in low glucose medium for 4 h. Cells were then treated with either low or high glucose for 2 h, and immunofluorescence was performed as described under “Experimental Procedures.” Over 100 cells in each treatment were scored as predominantly cytoplasmic, both cytoplasmic and nuclear, or predominantly nuclear by two observers. Results represent the means ± S.E. for three experiments. b, cells were treated with low (open triangles) or high glucose (closed squares) medium with the addition of leptomycin B at various time points as indicated. Immunofluorescence and quantification was performed as described above. Cells displaying either predominantly nuclear or both nuclear and cytoplasmic localization were combined and expressed as a percentage of the total cells.