FIGURE 4.

Leptomycin B does not inhibit the induction of ChREBP target genes in 832/13 cells. 832/13 cells were pretreated in low glucose RPMI medium for 4 h with or without leptomycin B (Lep) for the last 90 min. Cells from both experimental groups were then incubated with low or high glucose for 4 h. RNA was isolated and converted into cDNA using reverse transcriptase. mRNA levels of ChREBP target genes, thioredoxin-binding protein (Txnip) and aldolase B (AldoB), were measured by quantitative reverse transcription-PCR. mRNA levels in low glucose without the addition of leptomycin B were set to 1, and all values are normalized to this group. Values represent the mean ± S.E. of triplicate samples.