FIGURE 1.

Determination of the 5′ end of TER1 by primer extension. Oligonucleotide BLoli1116 (complementary to nucleotides 98-118) was radiolabeled and used for primer extension with total RNA, poly(A) enriched fraction, and RNA isolated from Trt1-Myc immunopurifications. No signal was observed with total RNA from a ter1Δ strain (lane 7). The three major 5′ ends are marked with arrows. The percentages next to the sequence indicate relative amount of RNA isolated from the Trt1-Myc sample starting at this position. Dideoxynucleotide sequencing (lanes 1-4) was performed with BLoli1116 and a cloned fragment of the ter1⁺ gene. Sequencing lanes are labeled for the strand representing the TER1 RNA.