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. Author manuscript; available in PMC: 2008 Aug 29.
Published in final edited form as: Biochemistry. 2006 May 2;45(17):5430–5439. doi: 10.1021/bi0525775

Figure 1.

Figure 1

Rhodopsin bleaching sequence near physiological temperatures in membrane. Some of the intermediates can be trapped after low-temperature photolysis, but those shown in italics, BSI (whose equilibrated mixture with Batho is sometimes called BL) and Meta I380, only build up appreciable concentrations near physiological temperatures (15, 23, 34). The time constants given are appropriate for membrane suspensions of rhodopsin near 20 °C. This general scheme also holds for detergent samples (such as DM) with the same time constants up to Lumi II formation. However, DM forward shifts both the Lumi II ⇄ Meta I380 and the Meta I480 ⇄ Meta II* equilibria so that little or no Meta I480 appears at high DM to rhodopsin ratios. Because the two Meta II forms are isochromic, their equilibrated mixture was originally referred to as metarhodopsin II. Meta II* is also called MIIa and it should be distinguished from the G protein activating form R*, also called MIIb.