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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Aging Cell. 2008 Mar 24;7(3):355–367. doi: 10.1111/j.1474-9726.2008.00393.x

Figure 3.

Figure 3

Increased levels of wild-type lamin A in primary human fibroblasts are sufficient to induce the formation of nuclear blebs. A. Fluorescence microscopy of DAPI stained cells show typical nuclei of HGPS fibroblasts and normal diploid fibroblasts expressing either flag-progerin, wild-type flag-lamin A, or GFP. White arrow in the bottom right panel shows nuclear blebs that are occasionally seen in normal fibroblasts. B. Quantitation of the cells with one or more nuclear blebs from passages 7 to 13. For each experiment, 300 cells were scored by two independent observers for nuclear blebs as described in Materials and Methods. Data shown for cells at passages 7 through 11 represent averages of two measurements per cell line while data for passage 13 represent mean +/− standard deviation of 3 measurements in two independent cell lines for each group.