FIG. 4.

Specific whole-cell uptake of [³H]NE in BACC (A) and MPC cells (B) infected with Ad.null. Forty-eight and 72 h after infection, cells were incubated with [³H]NE (50 nm/well) for 10 min at 37 C (total whole-cell uptake) or 4 C (nonspecific whole-cell uptake). Specific whole-cell uptake was calculated as the difference between total whole-cell uptake and nonspecific whole-cell uptake. Results were corrected for intracellular protein concentration measured in the cell lysates and are presented as mean ± sem of quadruplicates from four experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.