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. 2008 Jun 27;95(6):2849–2858. doi: 10.1529/biophysj.108.133629

FIGURE 3.

FIGURE 3

EPR spectra for the R1 spin label at hydrocarbon-facing sites 150, 152, 154, and 158 in β-strand 2 of the BtuB barrel reconstituted into POPC (gray trace) and DMPC (black trace), (A) below (17°C) and (B) above (28°C) the phase-transition temperature of DMPC. At each site, the spectra are virtually identical in either DMPC or POPC at 17°C, but differ at 28°C. The spectra indicate that R1 experiences more averaging in DMPC than in POPC in the liquid crystalline phase.