FIGURE 3.
EPR spectra for the R1 spin label at hydrocarbon-facing sites 150, 152, 154, and 158 in β-strand 2 of the BtuB barrel reconstituted into POPC (gray trace) and DMPC (black trace), (A) below (17°C) and (B) above (28°C) the phase-transition temperature of DMPC. At each site, the spectra are virtually identical in either DMPC or POPC at 17°C, but differ at 28°C. The spectra indicate that R1 experiences more averaging in DMPC than in POPC in the liquid crystalline phase.