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EPR spectra of R1 side chain incorporated into (A) outward-facing sites on strand 7, where arrow indicates a region on strand 7 with a highly positive (outward) curvature, (B) inward-facing sites on strands 2 and 3, and (C) outward facing sites on strands 2 and 3. Spectra B and C were previously described (33). Spin-labeled BtuB mutants were purified and reconstituted into POPC vesicles. (B) Labeled sites along strand 7.
