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Barrier heights for the unbinding and rebinding as a function of the applied force Fsp = KeffX0(t) calculated for different stiffness values: Keff = 10−1 N/m (dashed curve), 10−2 N/m (solid curve), and 10−3 N/m (dotted curve). Inset shows a total potential experienced by the pulled molecule. Parameter values: U0 = 15 kBT, Rc = 0.3 nm, b = 1.5.
