FIGURE 5.

Influence of pulse amplitude on the time course of fluorescence during and after electropermeabilization. The changes in fluorescence were monitored on a (A) 2 ms, (B) 80 ms, and (C) 8 s acquisition interval. CHO cells in suspension containing 100 μM of PI were exposed to a single rectangular 1 ms pulse with amplitudes of 350, 500, 650, and 800 V. The dashed line in A denotes the end of the pulse, and the horizontal dashed line in C is the baseline. Results shown on different acquisition intervals were obtained from different experiments. The background fluorescence was subtracted by the differential acquisition of the signal. Note different scale on the y axes.