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. 2008 Jun 13;95(6):3028–3035. doi: 10.1529/biophysj.108.132274

FIGURE 1.

FIGURE 1

(A) Panel of images shows the entropic swelling of an isolated H2B-EGFP nucleus digested with 0.5 mU/μL clostripain. The corresponding time points (minutes) are indicated. The scale bar is equal to 10 μm. (B) Expansion kinetics at different clostripain concentrations—1 mU/μL (squares), 2 mU/μL (circles), and 4 mU/μL (triangles)—normalized to the initial area in each case. (C) The fold change in cross-sectional area with respect to the initial ∼750 s after addition of the enzyme (or until the nucleus bursts), shown for two different clostripain concentrations: 0.5 mU/μL and 2 mU/μL. The mean and standard deviations for six different experiments each are plotted. (D) Nucleic acid digestions of isolated nuclei. Normalized area plots as in B are shown for 50 mU/μL (triangles), 500 mU/μL (circles) DNase 1, or 2 mg/mL RNase (squares). No entropic expansion is observed in any of the cases.