FIGURE 6.
Effect of bleaching on the thermal denaturation of cross-linked rhodopsin in disk membranes. Representative thermograms of cross-linked disk membranes at increasing percent bleach. (A) 16% dimeric rhodopsin at 0% bleach (solid curve), 40% bleach (dashed curve), and 57% bleach (dotted curve); (B) 34% dimeric rhodopsin at 0% bleach (solid curve), 28% bleach (dashed curve), and 60% bleach (dotted curve). Thermograms of cross-linked disk membranes were acquired at a 1.5°/min scan rate and smoothed over 25 points using Origin 5.0 software for clarity.