TABLE 1.

Orientation-related properties of the AlexaFluor dyes

	Site 42			Site 55
AF	θ	u	B	θ	u	B
1 AF488	61	0.96	−0.18	53	0.86	0.046
2 AF568	69	0.96	−0.37	69	0.96	−0.38
3 AF488	83	0.98	−0.61	47	0.90	0.22
4 AF568	74	0.95	−0.48	71	0.96	−0.42
5 AF488	72	0.97	−0.43	39	0.90	0.48

These properties were found during simulations in which they are attached to residue 42 or residue 55 of MscL.