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. 2008 Jun 6;95(6):2779–2791. doi: 10.1529/biophysj.108.130732

FIGURE 4.

FIGURE 4

Spreading (A) and compression (B) isotherms of BLES-albumin films with SP-A. Films were spread from an aqueous suspension of 5 mg/mL BLES with 2.5% SP-A and 20 mg/mL albumin. The amounts spread were usually 10 μL, unless high deposition pressure was required. To deposit a film at 45 mN/m, 15-μL samples were spread. The subphase and rate of compression are the same as in Fig. 1. A compression isotherm of BLES and SP-A films without albumin is shown as a reference. This film was prepared by spreading 10-μL 5 mg/mL BLES with 2.5% SP-A. For reasons of comparison, this compression isotherm is shifted to the right to coincide with the initial π of the compression isotherms of the films with albumin.