Table 1.
Treatment | MTT Reduction (% control) | ||
2 h | 3 h | 4 h | |
Eotaxin | |||
(10 ng/ml) | 84.6 ± 6.6 | 113.0 ± 6.1 | 91.0 ± 2.7 |
(100 ng/ml) | 113.8 ± 5.0* | 130.7 ± 2.3* | 127.3 ± 3.6* |
(1000 ng/ml) | 124.7 ± 5.0* | 138.4 ± 2.0* | 146.0 ± 11.8* |
RANTES | |||
(10 ng/ml) | 104.8 ± 1.6 | 128.8 ± 8.0* | 105.8 ± 1.0 |
(100 ng/ml) | 118.9 ± 2.8* | 133.4 ± 3.6* | 138.4 ± 6.0* |
(1000 ng/ml) | 117.4 ± 3.8* | 118.0 ± 1.0* | 147.0 ± 6.9* |
Eosinophil suspension (2 × 106cells/ml) was incubated for 2, 3 or 4 h with eotaxin (10–1000 ng/ml) or RANTES (10–1000 ng/ml). Each experiment was carried out in triplicate (n = 3). Results are expressed as MTT reduction (% control). *P < 0.05 compared to respective untreated cells.