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Activation of FRE-dependent transcription by the A kinase depends on Ste12p and Tec1p. Yeast strains YHUM120 (ras2), YHUM158 (ras2 ste20), YHUM175 (ras2 ste12), and YHUM167 (ras2 tec1) containing pRS426 (vector), YEplac195-TPK1 (2 μm TPK1), pRS426-TPK2 (2 μm TPK2), or pRS426-TPK3 (2 μm TPK3) were assayed for FRE(Ty1)::lacZ expression levels by measuring β-galactosidase activity. Units are nanomoles per minute per milligram. Bars depict means of three independent measurements.
