Table 2.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pras1Δ::HIS3 | Cassette for full deletion of RAS1-open reading frame | This study |
| pras2Δ::URA3 | Cassette for full deletion of RAS2-open reading frame | M. Wigler |
| pste20Δ::HIS3 | Cassette for full deletion of STE20-open reading frame | This study |
| pste12Δ::HIS3 | Cassette for full deletion of STE12-open reading frame | This study |
| ptec1Δ::HIS3 | Cassette for full deletion of TEC1-open reading frame | This study |
| pRS316-RAS2 | 3-kb fragment containing RAS2 in pRS316 | This study |
| YCplac22-RAS2 | 3-kb fragment containing RAS2 in YCplac22 | This study |
| YEplac112-RAS2 | 3-kb fragment containing RAS2 in YEplac112 | M. Wigler |
| YCp50-RAS2Val19 | 3-kb fragment containing RAS2Val19 in YCp50 | M. Wigler |
| YCplac22-RAS2Val19 | 3-kb fragment containing RAS2Val19 in YCplac22 | This study |
| YCplac22-RAS2Gly41 | 3-kb fragment containing RAS2Gly41 in YCplac22 | This study |
| YCplac22-RAS2Asn45 | 3-kb fragment containing RAS2Asn45 in YCplac22 | This study |
| YCplac22-RAS2Val19Gly41 | 3-kb fragment containing RAS2Val19Gly41 in YCplac22 | This study |
| YCplac22-RAS2Val19Asn45 | 3-kb fragment containing RAS2Val19Asn45 in YCplac22 | This study |
| pRS314-RAS2ΔC | 3-kb fragment containing RAS2 with C-terminal deletion in pRS314 | This study |
| pRS314-R2N-R1C | 3.5-kb fragment containing RAS2-RAS1 chimeric gene in pRS314 | This study |
| YCplac22-R2p-R1orf | 3.6-kb fragment containing RAS2-promoter fused to RAS1-ORF in YCplac22 | This study |
| YEplac112-RAS1 | 3-kb fragment containing RAS1 in YEplac112 | This study |
| pRS316-GAL-CDC42Val12 | 2.5-kb fragment containing CDC42Val12 fused to GAL1-10-promoter in pRS316 | This study |
| pRS316-GAL-CDC42Leu61 | 2.5-kb fragment containing CDC42Leu61 fused to GAL1-10-promoter in pRS316 | This study |
| pRS426-STE20 | 4.9-kb fragment containing STE20 in pRS426 | Mösch et al., 1996 |
| pYGEX-STE20 | GST-STE20 fusion gene under control of the GAL1-10-promoter | Roberts et al., 1997 |
| B2616 | 5.6-kb fragment containing STE11-4 in YCp50 | G. Sprague |
| pRS426-STE12 | 5-kb fragment containing STE12 in pRS426 | This study |
| B2065 | GAL1-10::STE12 fragment in URA3 marked 2-micron plasmid | B. Errede |
| pRS202-TEC1 | 6.5-kb fragment containing TEC1 in pRS202 | Mösch and Fink, 1997 |
| pGAL-TEC1 | TEC1-cDNA fused to GAL1-10-promoter in pRS316 | This study |
| YEplac195-TPK1 | 2.5-kb fragment containing TPK1 in YEplac195 | This study |
| pRS426-TPK2 | 5.3-kb fragment containing TPK2 in pRS426 | This study |
| pRS426-TPK3 | 3.9-kb fragment containing TPK3 in pRS426 | This study |
| YIpFRE(Ty1)::lacZ | Integrative FRE(Ty1)::lacZ reporter construct | This study |
| YIp356R-FG(Ty1)::lacZ | Integrative FG(Ty1)::lacZ reporter construct | S. Kron |