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. 2008 Aug 8;4:30. doi: 10.1186/1746-6148-4-30

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Copyright © 2008 Moore et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Monitoring the effect of pyrrole exposure on equine plasma fibrinogen using western blotting. Equine plasma was reacted with varying concentrations of DHM for 60 min at room temperature. Control plasma was treated with a reaction mixture that was complete apart from DHM. Samples were analysed by 1-D SDS-PAGE and proteins were blotted onto nitrocellulose membranes and blocked overnight before probing with an anti-equine fibrinogen antibody. Blots were then incubated with an alkaline phosphatase linked secondary antibody and developed. Migration of fibrinogen α-chain was evident following the exposure of a large excess of pyrrole.