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. 2008 Aug 7;8:77. doi: 10.1186/1471-213X-8-77

Figure 4.

Figure 4

XCIRP is a Tcf-subtype specific target gene. (A) Anterior view of in situ hybridization reveals that depletion of XTcf-3 by morpholino injection (Tcf3Mo), but not depletion of XTcf-4 (Tcf4Mo) or XLef-1 (LefMo1,2) results in an absence of XCIRP staining at the injected side (asterisk). (B) XCIRP expression in Tcf3Mo injected embryos can be restored by co-injection of XTcf-3 and XLef-1 mRNA. The asterisks mark the injected side. (C, D) The rescue of XCIRP expression by XTcf-3 is dose dependent and Tcf-subtype specific. In (C): the indicated amount of N-terminal myc tagged XTcf-3mRNA was co-injected with two picomoles of Tcf3Mo. In (D): 1000 pg of the indicated Lef/Tcf mRNA or 300 pg β-catenin mRNA were coinjected with two picomoles of Tcf3Mo. Given is the percentage of embryos showing reduced or absent XCIRP expression at the injected side. n: number of analyzed embryos.