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ASV IN mediated import is inhibited by excess histone H1. The import of labeled histone H1, (GST-IN(1–286), and the Impβ binding domain fused to GFP (IBB-GFP) was examined in the absence (top) and presence (bottom) of excess unlabeled histone H1. Incubations were for 30 min and all exposure times were equivalent.
