Fig. 5.
Isolated mitochondria were treated in the presence or absence of proteinase K (PK). MitoFluor Red positive mitochondria were selected and gated for by flow cytometry. A significant inhibition of Aβ/α-Tom20/PK, Aβ/α-Tom40/PK, and Aβ/α-Tom70/PK (*, P < 0.05, *, P < 0.05, **, P < 0.01) as compared to Aβ/PK treated mitochondria is shown (A–C). Pretreatment of mitochondria with VDAC antibody, valinomycin (Val) or cyclosporine A (CsA) had no statistically significant effects on Aβ1-40 import (D–F).