Fig. 1.

Recombinant calreticulin (CRT) fragments are recognized by a rabbit anti-CRT anti-serum. Nitrocellulose-blotted recombinant 6xHis-tagged quarters and halves of human CRT and, for control purposes, recombinant full length CRT were exposed to a polyclonal rabbit anti-CRT anti-serum. Specific antibody binding was detected with peroxidase-labeled anti-rabbit antibodies and visualized using chemiluminescence detection system by autoradiography. Molecular weights are indicated in the left margin. The individual quarters of CRT corresponded with 1–103 amino acids, 104–206 amino acids, 207–309 amino acids and 310–400 amino acids; corresponding halves of CRT corresponded with 1–206 and 207–400 amino acids.