Fig. 5.

Aquaporin 5 (AQP5) knock-out reduced the level of sera ovalbumin (OVA)-specific immunoglobulin E (IgE) (*P < 0·01). OVA-specific IgE levels in sera from wild-type mice were higher than those from AQP5^−/− asthma models. Coating plates overnight with 10 µg/ml OVA grade, serial dilutions of sera were applied, followed by biotin-conjugated anti-mouse IgE. A serum pool of OVA-sensitized mice was used according to the internal laboratory standard.