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. Author manuscript; available in PMC: 2008 Aug 29.
Published in final edited form as: Environ Sci Technol. 2006 Nov 1;40(21):6852–6858. doi: 10.1021/es060811o

FIGURE 1.

FIGURE 1

Lead concentrations in experimental and control aquaria during chronic study of tissue lead distribution in adult fathead minnows. Aliquots of lead nitrate stock solution (0.26 - 1.00 mL in water) were repeatedly added to tanks B, C and D to attain “low”(B), “moderate”(C) and “high” (D) doses. The majority of aquarium D fish died during the initial week. Lead concentration in the control aquarium (A) remained near the limit of detection (1 ppb, HachLeadTrakR method) throughout the study. For aquarium B, ten additions were made, using either 0.78 mg/mL as Pb (the initial 5 additions ranged from 0.51 - 0.78 mg Pb)) or 7.8 mg/mL as Pb (the subsequent 5 additions ranged from 2.03 - 4.5 mg Pb) of Pb(NO3)2 stock solution in dH2O. The cumulative amounts of Pb added after 7 and 21 days were 3.36 mg and 18.89 mg, respectively. For aquarium C, seven additions were made, using 10 mg/mL as Pb of Pb(NO3)2 stock solution. The amount of lead per addition ranged from 4.4 mg to 8.78 mg. The cumulative amounts of Pb added after 7 and 26 days were 26.33 and 43.93 mg as Pb. For aquarium D, five additions were made, using 7.8 mg/mL as Pb of Pb(NO3)2 stock solution. The amount of lead per addition ranged from 5.1 mg to 7.8 mg. The cumulative amount of Pb added after 7 days was 33.6 mg as Pb.