Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Mos-induced activation of p42MAPK does not inhibit DNA replication in Xenopus egg extracts. Buffer, Mos(KM), or Mos(WT) was added to CHX extracts and then incubated at room temperature for 40 min. Demembranated sperm nuclei (600 nuclei/μl) and [α-32P]dATP (0.2 μCi/μl) were added to each reaction, and samples taken at 0, 0.5, 1, and 2 h of incubation at room temperature. Samples were analyzed by electrophoresis on 0.8% agarose gels (see MATERIALS AND METHODS).
