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Resolution factors obtained between two solutes related by a distribution constant ratio equal to two or ΔLog KD = 0.3. Left curve: elution mode; right curve: extrusion mode. VC = 120 mL, VM = 50 mL, VS = 70 mL, VCM = 254 mL and N = 900 plates. The last solutes have left the column at volume VCM + VC = 254 + 120 = 374 mL, but at this point the resolution factor is nil.
