Table 2.

Retention volumes, peak widths and resolution factors of hypothetical solutes

KD	VR	Wb	Rs
0.1	57	7.60
0.2	64	8.53	0.86
0.4	78	10.4	1.46
0.8	106	14.1	2.27
1.6	162	21.6	3.12
3.2	274	36.4	3.84
6.4	334.31	12.05	2.50
12.8	354.16	8.52	1.93
25	363.84	6.10	1.32
50	368.92	4.31	0.97
100	371.46	3.05	0.69
200	372.73	2.16	0.49
400	373.37	1.53	0.34
800	373.68	1.08	0.24
1600	373.84	0.76	0.17

Values in bold correspond to the extrusion step. V_C = 120 mL, V_M = 50 mL, V_S = 70 mL, V_CM = 254 mL and N = 900 plates. The limit between elution and extrusion occurs for K_D = V_CM/V_S = 254/70 = 3.63 and V_R = V_CM + V_M = 304 mL Total elution is V_CM + V_C = 374 mL.