Table 3.
Elution-Extrusion of an Alkylbenzene and a Steroid Mixture
| compounda | KD | VR exp. | VR ref.17 | deviation | VR calc. | Deviation |
|---|---|---|---|---|---|---|
| Hydrodynamic 3-coil CCC column, VC = 175 mL, VM = 80 mL, VS = 95 mL, VCM = 270 mL | ||||||
| benzene | 2.0 | 268 | 273 | +1.9% | 270 | +0.7% |
| toluene | 3.0 | 352 | 346 | −1.7% | 355 | +0.9% |
| ethylbenzene | 4.0 | 380 | 386 | +1.6% | 378 | −0.5% |
| butylbenzene | 7.7 | 412 | 444 | +7.8% | 410 | −0.5% |
| octylbenzene | 31 | 436 | 495 | +13.5% | 436 | 0% |
| Hydrostatic CPC column, VC = 101 mL, VM = 21 mL, VS = 80 mL, VCM = 199 mL | ||||||
| benzene | 2.0 | 184 | 183 | −0.5% | 181 | −1.6% |
| toluene | 3.0 | 228 | 232 | +1.7% | 233 | +2.2% |
| butylbenzene | 7.7 | 252 | 287 | +14% | 274 | +8.7% |
| octylbenzene | 31 | 268 | 312 | +16% | 293 | +9.3% |
| Hydrodynamic 1-coil CCC column, VC = 52.2 mL, VM = 21 mL, VS = 31.2mL, VCM = 33 mL19 | ||||||
| prednisone | 0.12 | 24.6 | - | 24.7 | +0.4% | |
| prednisolone | 0.56 | 38.6 | - | 38.5 | −0.2% | |
| testosterone | 1.4 | 62.8 | - | 61.6 | −1.9% | |
| estrone | 4.6 | 78 | - | 78 | 0% | |
| cholesterol | 40 | 84.6 | - | 84.4 | −0.2% | |
a
alkylbenzene were separated with the liquid system heptane/methanol/water 5/4/1 v/v with the aqueous lower mobile phase and the heptane lighter stationary and extruding phase. Steroids were separated with the heptane/ethyl acetate/methanol/water 5/6/5/6 v/v liquid system with the aqueous lower mobile phase and the organic lighter stationary and extruding phase.