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. Author manuscript; available in PMC: 2008 Aug 30.
Published in final edited form as: Nano Lett. 2007 May 15;7(6):1757–1763. doi: 10.1021/nl070828k

Figure 2.

Figure 2

Non-Denaturing Gels Demonstrating Formation of Half-Tube Components. (a) Blunt-ended versions of the BTX molecules phased opposite each other. A marker lane containing 100 nt fragments is labeled M. Lanes 2 and 3 contain the two components and lane 1 shows their cohesion as a clean band. (b) Blunt-ended versions of the molecules that are offset by a half length. Both lanes contain a well-formed single band. (c) The subcomponents of a blunt-ended BTX molecule. The contents of individual lanes are indicated above them, with a ‘1’ indicating the presence of the component. A 10 nt fragment marker lane is at right. (d) Formation of the 8HB from the 4HB molecule. A 100 nt marker lane is labeled ‘M’. Lane 1 contains the individual 4HB molecule without lateral or terminal single strands. Lane 2 contains two molecules joined only at point A (8HB-A), and lane 3 contains two molecules joined only at point B (8HB-B). Both lanes contain various breakdown products. Lane 4 contains the complete 8HB without sticky ends. It is cleanly formed.