Table 1.
Protein Concentration | Kssω (M−1) | τ(s) |
---|---|---|
[*I]=4 nM | 7.9 ± 1.2×109 | 116.1 ± 21.0 |
[*I]=4 nM + [UvsY]=16 nM | 1.4 ± 0.2×108* | 128.7 ± 8.0 |
[*I]=4 nM + [UvsY]=32 nM | 5.9 ± 0.9×108* | 165.0 ± 10.0 |
[*I]=4 nM + [UvsY]=50 nM | 1.8 ± 0.3×108* | 210.2 ± 21.0 |
[*I]=4 nM + [UvsY]=80 nM | 8.8 ± 0.1×107* | 278.8 ± 15.0 |
[*I]=4 nM + [UvsY]=150 nM | 4.3 ± 0.7×107* | 363.0 ± 90.0 |
All measurements were performed in 10 mM Hepes pH 7.5, 50 mM [Na+]. Data are reported as mean ± standard error for n≥4.
Apparent binding constants