Table 1. Primers used in this study.
Primer | Sequence |
---|---|
Rv1086UP* | CAT ATG GAG ATC ATC CCG CCG CGG CTC A |
Rv1086RP* | AAG CTT TCA CCT GCC GTA GCT GCG ATG CCT |
Rv1086L84AUP † | CGT CTA TGC TCT GTC CAC CGA AAA CCT GC |
Rv1086L84ARP † | TGG ACA GAG CAT AGA CGG TGG CCA GTT |
Rv1086L84A,L85FUP † | ACC GTC TAT GCT TTC TCC ACC GAA AAC CTG C |
Rv1086L84A,L85FRP † | TTC GGT GGA GAA AGC ATA GAC GGT GGC CAG TTC |
Genomic DNA was used as the template
Plasmid containing Rv1086 was used as the template