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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: J Mol Biol. 2008 Jul 1;381(1):129–140. doi: 10.1016/j.jmb.2008.05.060

Table 1. Primers used in this study.

Primer Sequence
Rv1086UP* CAT ATG GAG ATC ATC CCG CCG CGG CTC A
Rv1086RP* AAG CTT TCA CCT GCC GTA GCT GCG ATG CCT
Rv1086L84AUP CGT CTA TGC TCT GTC CAC CGA AAA CCT GC
Rv1086L84ARP TGG ACA GAG CAT AGA CGG TGG CCA GTT
Rv1086L84A,L85FUP ACC GTC TAT GCT TTC TCC ACC GAA AAC CTG C
Rv1086L84A,L85FRP TTC GGT GGA GAA AGC ATA GAC GGT GGC CAG TTC
*

Genomic DNA was used as the template

Plasmid containing Rv1086 was used as the template