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. 1999 May;10(5):1337–1351. doi: 10.1091/mbc.10.5.1337

Figure 2.

Figure 2

Cloning and characterization of APG7. (A) WT (wild-type, SEY6210), apg7 (THY193), apg7Δ (VDY1), and the apg7Δ strain transformed with single copy (CEN, pAPG7(414)) or multicopy (2 μ, pAPG7(424)) plasmids encoding APG7 were grown to log phase in SMD. Protein extracts were prepared and analyzed by immunoblot using antiserum to API as described in MATERIALS AND METHODS. The positions of precursor and mature API are indicated. The APG7 gene complements the precursor API accumulation phenotype of the apg7Δ mutant. (B) WT (wild-type, SEY6210), apg7Δ (VDY1), and the apg7Δ strain transformed with the APG7 centromeric plasmid pAPG7(414) were grown in SMD and transferred to SD-N as described in MATERIALS AND METHODS. Aliquots were removed at the indicated times and spread onto YPD plates in triplicate. Numbers of viable colonies were determined after 2–3 d. The APG7 gene complements the starvation-sensitive phenotype of the apg7Δ mutant.