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. 2008 Sep 12;3(9):e3204. doi: 10.1371/journal.pone.0003204

Figure 3. P.g and/or high fat diet increases atherosclerotic lesions in proximal aorta of ApoE+/−-TLR2+/+ mice when compared to ApoE+/−-TLR2+/−, and ApoE+/−-TLR2−/− mice.

Figure 3

Microscopic cross-sections (10 µm) of the proximal aortic root were stained with Sudan IV and counterstained with hematoxylin to reveal lipid deposition, which was quantified by digital morphometry. (3A–D): results from mice maintained on a standard chow diet and inoculated weekly with P. g (CP). (3E–H): results from mice maintained on a high fat diet and inoculated weekly with vehicle (HS) (normal saline). (3I–L) results from mice maintained on a high fat diet and inoculated weekly with P. g (HP). (3D, H, l): data are presented graphically as percentage of total lumen of the proximal aorta occupied by lesions after 24 weeks of injections. Values represent means±SD; *p<0.05 between ApoE+/−-TLR2+/+ mice and ApoE+/−-TLR2+/− mice; **p<0.05 between ApoE+/−-TLR2+/+ mice and ApoE+/−-TLR2−/− mice in the same condition and maintained on the same diet. Abbreviations are as defined in the text. Photomicrographs shown are representative images obtained at the end of the 24 week treatment period. Original magnifications 20×. Scale bar represents 0.5 mm.