Figure 6. Decrease in CLPs, NKPS and Block in NKP progression.

B6 mice were sublethally irradiated with 500 Rad prior to EL4 or PBS injection. Twenty-four hours later 5FU treated bone marrow from congenic CD45.1 mice was injected into control or EL4-bearing mice. Three weeks later bone marrow was harvested to evaluate NK cell progenitors. Donor NK cells were identified by an anti-CD45.1. A. Diagram of experiments. B. FACs profile of CD45.1⁺ NK1.1⁺ cells. Data shown are from gated CD45.1⁺CD3⁻ cells. The numbers shown in the panels are means and SEM, involving a total of 5 mice per group. C. Absolute number of CD45.1⁺ NK cells. D. Absolute number of donor B cells. E. FACS profile of donor CLP, characterized as CD45.1⁺Lin⁻CD127⁺cKit^int. Data are representative of 5 mice per group. F. Absolute number of CD45.1⁺ and CD45.1⁻ CLP. Data shown are means and SEM, involving a total of 5 mice per group.