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. Author manuscript; available in PMC: 2009 Jul 16.
Published in final edited form as: Mol Cell Endocrinol. 2008 Apr 22;289(1-2):16–22. doi: 10.1016/j.mce.2008.04.009

Fig. 1.

Fig. 1

D2 expression in human medullary thyroid carcinoma (MTC). A. D2 mRNA levels in MTC and corresponding normal tissue. Individual data points for each sample are depicted. Results are expressed in arbitrary units. B. An illustrative RT-PCR of D2 products in tumor (T) and surrounding normal tissue (N) from cases 3, 5-7, 9 and 10 (Table 2; 1.5% agarose gel stained by ethidium bromide). C. D2 enzyme activity in MTC and corresponding normal tissue. Individual data points for each sample are depicted. D2 activity was measured as the release of I- from 1nM T4 as substrate. D. Double-reciprocal plot of D2 activity in normal thyroid tissue (solid circles) and MTC (open circles). D2 activity was measured using different concentrations of 125I-T4 as described in Materials and Methods.