a, RT-PCR and Western blot analysis of AMCase and Ym2 in the lung following N. brasiliensis infection. b, FACS analysis 1 day after 2 intranasal chitin doses to 4get mice. Eosinophil (GFP+27 Siglec F+30) and basophil (GFP+27, IgE+ cKit−27) gates shown. c, Kinetics of chitin-induced lung eosinophils. d, FACS of peritoneal cells 2 days after i.p. chitin. Eosinophils and mast cells (GFP+IgE+cKit+) gated. e, Kinetics of chitin-induced peritoneal cells. Data representative of 2–3 independent experiments.