Skip to main content
. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: Nature. 2007 Apr 22;447(7140):92–96. doi: 10.1038/nature05746

Figure 2. AMCase prevents chitin-induced eosinophil and basophil recruitment.

Figure 2

a, Designated mice received intranasal chitin and lung eosinophils were quantitated by flow cytometry. b, Chitin or chitin preincubated with recombinant AMCase or Ym2 was administered to 4get mice and recruitment of lung eosinophils and basophils was determined. c, Chitin was preincubated with PBS, recombinant AMCase, or recombinant mutant enzymatically-inactive AMCase (AMCmut) and given to 4get mice. d, AMCase expression in SPAM transgenic mice assessed by RT-PCR and Western blot analysis of lung tissues from naïve, day 9 N. brasiliensis-infected wild-type and naïve SPAM mice. e, SPAMx4get transgenic mice (SPAM) or wild-type 4get mice were given intranasal chitin. * p<0.05, ** p<0.01, and *** p<0.001 P-values were determined using Student’s t-test. Error bars represent standard deviations. In FACS plots, numbers represent percentage of gated cells from total live cells. Shown is representative of three independent experiments, with n=3–4 per group.