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Conformational heterogeneity revealed by comparison of anisotropic couplings measured for OmpA in a 50+M gel. A) spectral region of 1H-15N TROSY-HSQC showing major and two minor peaks for Tyr129; B and C) comparison of anisotropic 1JHN couplings for three peaks of Tyr129; D and E) comparison of 1JC′Cα couplings for major (D) and minor peak (E) for Met53; F and G) comparison of 1JNC′ couplings for Met53.
