Figure 3.

Complete VPF/VEGF₁₆₅ 3′ UTR cDNA sequence and potential regulatory elements. (A) Complete 3′ UTR cDNA sequence beginning with base 1 as the first base after the translation stop codon in the coding region. The polyadenylation site at the 3′ end consists of 13 adenosine residues but is not preceded by a consensus AAUAAA sequence. Two consensus internal polyadenylation sequences are encircled. Two destabilizing elements with the consensus sequence 5′-UUAUUUA(U/A)(U/A)-3′ are indicated (solid boxes). Five underlined sequences show partial consensus sequences that include the AUUUA sequence. Broken boxes indicate AU-rich elements consisting of at least five AU pairs in a row (AU 1–4). The human VPF/VEGF₁₆₅ 3′ UTR sequence is accessible in GenBank accession no. AF022375. (B) Schematic map of VPF/VEGF₁₆₅ FL cDNA and the RNA transcripts used in this study. The numbers indicate base location in the 3′ UTR, and the numerical bar on the bottom represents the 3′ UTR sequence given in A.