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. 1998 Feb;9(2):469–481. doi: 10.1091/mbc.9.2.469

Figure 5.

Figure 5

Hypoxia-inducible RNA–protein complexes form with the VPF/VEGF AU-rich 3′ HSR sequence in M21 cells. (A) RNA binding/EMSA was performed with an RNA transcript probe (3′ HSR). RNA–protein complexes were detected in the presence of both RNase T1 and RNase A. Orientation-specific RNA–protein complexes were detected by using sense (S) and antisense (AS) 3′ HSR probes and 24-h normoxic (N) and hypoxic (H) extracts. RNase-resistant complexes are indicated by arrows. Note the hypoxic induction of the faster mobility complex. (B) RNA–protein complexes competed with sense but not antisense RNA containing the 3′ HSR sequence. A 3′ HSR probe was used to perform RNA binding/EMSA with M21 normoxic cell extract without (−) and with a 50-fold excess of sense (S) or antisense (AS) 3′ HSR or 3′ 0.5-kb transcripts.