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Bcr-Abl and mTORC1 induce the formation of eIF4F in K562 cells. (a) K562 cells treated with imatinib with or without rapamycin (10 ng/ml) for 24 hrs. Cap-bound proteins were brought down by incubation with m7-GTP-Sepharose. The amount of cap-bound eIF4E and 4E-BP1 was then assessed by immunoblot with the appropriate antibodies. (b) The amount of cap-bound eIF4G and whole cell eIF4G was examined in K562 cells. Anti-c-Abl was used to assess equal loading for whole cell lysates.
