FIG. 3.

Effect of MnCl₂ on LHRH release (A) and NOS activity (B) from MBH incubated in medium containing Ca⁺² (1.25mM) or incubated in Ca⁺² free medium. Note that the MnCl₂-stimulated increases in both LHRH and NOS activity did not occur in absence of Ca⁺² in the incubation medium. *p < 0.05 and **p < 0.01 versus respective control. Values represent mean ± SEM.