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Effect of MnCl2 on GABA release (A) and the action of bicuculline (B), a GABAA receptor antagonist, on MnCl2-stimulated LHRH release from MBH in vitro. Note that both GABA and LHRH release were increased by MnCl2, and that bicuculline enhanced MnCl2-induced LHRH secretion but had no effect when incubated alone. *p < 0.05, and ***p < 0.001 versus control. Values represent mean ± SEM. The − or + symbols indicate the absence or presence of MnCl2 and bicuculline in the incubation medium.
