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The guanine-nucleotide binding site on human TGase-2. (A) TGase-2 residues which interact with the guanine nucleotide (GDP-shown in blue) are represented by ball and stick. (B) Sequence alignments using ClustalW (59) of the guanine nucleotide-binding sites of TGase-2 and other members of the transglutaminase family that bind guanine nucleotides. The numbered residues in orange represent the sites mutated in TGase-2.
