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. Author manuscript; available in PMC: 2008 Dec 25.
Published in final edited form as: Biochemistry. 2007 Dec 1;46(51):14819–14829. doi: 10.1021/bi701422h

Figure 6.

Figure 6

GTP-binding-defective TGase-2 mutants induce cell death. (A, C) NIH 3T3 cells and (B, D) Hela cells were transiently transfected with vector alone or with one of the indicated Myc-tagged TGase-2 constructs and maintained in complete medium (medium containing 10% serum). NIH 3T3 cells were fixed either 24 hours (A) or 36 hours (C) after transfection, while the Hela cells were fixed 36 hours (B) or 60 hours (D) post-transfection. Immunofluorescence using anti-Myc antibody and DAPI (to stain nuclei) was performed on the cells, and nuclear condensation or blebbing was used to detect cell death. The average percentage of cell death was plotted for each of the conditions and the error bars indicate standard deviations.