Figure 2. Resistance to heat shock of HepG2 cells cultured with serum collected at baseline and serum collected at the end of the diet regimen from FEAST and CALERIE participants.

HepG2 cells were cultured for 24 hrs in 96-well culture plates in culture media supplemented with 10% human serum. Plates were then either subjected to a 1 hr incubation period at 45°C or left under normal culture control conditions. 24 hrs after heat shock treatment, surviving cells were determined using a CCK-8 soln. Graph shows an increased resistance to heat shock in cells cultured with FEAST serum, * = p<0.0005 (A) and no changes in cells cultured in serum form all groups of the CALERIE study (B).